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The antimicrobial activities of the novel actinomycete Streptomyces flavoviridis were investigated. The 16S rDNA gene sequence of S. flavoviridis showed high homology (98.5%) with Streptomyces parvulus, and the ANIb value between S. flavoviridis and S. parvulus was 95.22%. The morphological characteristics of S. flavoviridis were similar to those of S. parvulus. Minimum inhibitory concentrations (MICs) of the novel actinomycete against 5 representative Gram-positive and negative bacteria and the antifungal activity were evaluated. The results indicated that S. flavoviridis produced inhibitory substances against all tested bacteria and fungi at concentrations of >20 mg/ml. Production of the antifungal substance was high at 25 °C and 42 °C, but was significantly reduced at 25 °C when treated with n-butyl alcohol. Finally, the actinomycete was isolated from a deep-sea sediment sample. Further evaluation of the compounds produced by this new actinomycete will provide useful information 0b46394aab